Device

Part:BBa_K2082116:Design

Designed by: Carsten Hain   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-14)


dnaQ926 under control of a modified Pbad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1283
    Illegal AgeI site found at 1772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Strong genome wide mutator dnaQ926s mutagensis capabilities should be assessed.


Source

cloning of BBa_K2082110 downstream of BBa_K2082112

References